[Nucleotides and mechanical capacity of the sartorius muscle of frogs intoxicated by idoacetate or fluorodinitrobenzene and briefly tetanized].
(1967) Journal de Physiologie — Vol. 59, n° 4 Suppl, p. 454 (1967)
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- Abstract
- Sartorius muscles of Rana temporaria were tetanized for one second at 0°C. in N2, either at constant length, or with a constant speed shortening during the second half of the tetanus, and frozen immediately after the last stimulus. Four-nucleotides (ATP, ADP, AMP and IMP) were determined by thin-layer chromatography of the trichloroacetic extracts followed by a spectrophotometric measurement. All muscles are poisoned for one hour at 0°C either by 0.4 mM iodoacetate (IAA), which blocks glycolysis, or by fluoro-2,4'-dinitrobenzene, 8 mM (FDNB), which blocks creatine kinase. FDNB decreases the production of maintenance heat hB, without influencing the production of labile heat hA (AUBERT, 1964), which leads to the conclusion that a hydrolysis of ATP corresponds to this labile heat hA.
Citations
Maréchal, G., & Beckers-Bleukx, G. (1967). [Nucleotides and mechanical capacity of the sartorius muscle of frogs intoxicated by idoacetate or fluorodinitrobenzene and briefly tetanized]. Journal de Physiologie, 59(4 Suppl), 454. https://hdl.handle.net/2078.5/214205 (Original work published 1967)