Protein-polyelectrolyte complexes for the surface immobilization of bioactive proteins
(2019)
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- Authors
- Supervisors
- Dupont, Christine
- Abstract
- The immobilization of proteins to impart specific functions to surfaces is topical for chemical engineering, healthcare and diagnosis. Layer-by-Layer (LbL) self-assembly is one of the most used method to immobilize macromolecules on surfaces. However, when it comes to proteins, the promises of versatility, simplicity and universality that the LbL approach holds are unmet due to the heterogeneity of protein properties. This thesis aims at demonstrating that protein surface properties can be standardized via their complexation with polyelectrolytes (PEs), and that the resulting protein-polyelectrolyte complexes (PPCs) can be used as building blocks for LbL assembly. The results show that using PPCs, LbL assembly becomes independent of the protein electrical properties. In particular conditions, proteins from PPCs serve as a sacrificial element to build layers with specific properties. When compared to the LbL assembly of bare proteins, the PPCs-based method is shown to maintain a higher protein bioactivity and to immobilize larger protein amounts, which results in a higher total bioactivity. Finally, the method is translated to the creation of a dressing on which an antimicrobial peptide is immobilized thanks to the use of PPCs. The LbL assembly of PPCs thus holds much promises in terms of universality. It also allows to create new functionality and structures at interfaces, which will be beneficial for the design of drug delivery systems, biomaterials and sensors.
- Affiliations
Citations
vander Straeten, A. (2019). Protein-polyelectrolyte complexes for the surface immobilization of bioactive proteins. https://hdl.handle.net/2078.5/96446