Impedimetric gold nanoparticle lateral flow test for pathogen detection
(2023)
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Details
- Authors
- Supervisors
- Raskin, Jean-Pierre
- Abstract
- Climate change and the expanding global population are placing huge pressure on our planet's natural resources. Safety of water resources and food production chains necessitate access to frequent and reliable data on pathogenic infections. The recent outbreaks highlighted the need for early diagnostic tools to screen pathogens, combining accuracy and rapidity with simplicity and accessibility. This thesis investigates how rapid diagnostic tests, such as the lateral flow assays (LFA), take advantage of porous paper-like membranes to develop highly specific colorimetric biosensors for the diagnostic of pathogens (Bacillus cereus) in a user-friendly and rapid (15 min) format. Then, this work tackles the challenges presented by colorimetric LFAs (low sensitivities) by exploring how reusable electrode systems can be added to LFAs towards improved detection performance. By means of a multidisciplinary approach, this thesis offers a complete characterization of the LFAs in order to support the future research of LFAs for pathogen diagnostics. The biosensor technologies developed in this thesis are not neutral. Many aspects condition their long-term use, such as the impacts associated with their production, dissemination, and end-of-life. Therefore, an eco-design approach is proposed early-on in the research process to anticipate the impacts of the design and manufacturing process of the pathogen biosensors.
- Affiliations
Citations
Le Brun, G. (2023). Impedimetric gold nanoparticle lateral flow test for pathogen detection. https://hdl.handle.net/2078.5/234116