Initial step of virus entry: virion binding to cell surface glycans
(2020) Annual Review of Virology — (2020)
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- Abstract
- (en) Virus infection is an intricate process that requires the concerted action of both viral and host cell components. Entry of viruses into cells is initiated by interactions between viral proteins and cell surface receptors. Various cell surface glycans function as initial, usually low-affinity attachment factors, providing a first anchor of the viruses to the cell surface and further facilitate high-affinity binding to virus-specific cell surface receptors, while other glycans function as specific entry receptors themselves. It is now possible to rapidly identify specific glycan receptors using different techniques, to define atomic-level structures of virus-glycan complexes and to study these interactions at the single-virion level. This review gives a detailed overview of the role of glycans in viral infection and highlights experimental approaches to study virus-glycan binding along with specific examples. In particular, we highlight the development of the atomic force microscope to investigate interactions with glycans at the single-virion level directly on living mammalian cells offering new perspectives to better understand virus-glycan interactions in physiologically relevant conditions.
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Köhler, M., Delguste, M., Sieben, C., Gillet, L., & Alsteens, D. (2020). Initial step of virus entry: virion binding to cell surface glycans. Annual Review of Virology. Accepted/in-press. https://hdl.handle.net/2078.5/96923 (Original work published 2020)