Immobilisation of glucose oxidase within metallic nanotubes arrays for application to enzyme biosensors.
(2003) Biosensors and Bioelectronics — Vol. 18, n° 7, p. 943-951 (2003)
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- Abstract
- Arrays of nanoscopic gold tubes were prepared by electroless deposition of the metal within the pores of polycarbonate particle track-etched membranes (PTM). Glucose oxidase (Gox) was immobilised onto preformed self-assembled monolayers (SAMs) (mercaptoethylamine or mercaptopropionic acid) on electroless gold via cross-linking with glutaraldehyde or covalent attachment by carbodiimide coupling. The effectiveness of the different steps in the Gox immobilisation procedure was assessed by contact angle measurements, cyclic voltammetry and X-ray photoelectron spectroscopy. The enzyme loading was estimated by radioactivity measurements. The sensitivity to beta-glucose of these different biosensors has been evaluated. Glucose responses as large as 400 nA mM(-1) cm(-2) have been obtained. To our knowledge, this sensitivity value is amongst the highest values reported in the literature for comparable biosensor systems.
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Delvaux, M., & Demoustier, S. (2003). Immobilisation of glucose oxidase within metallic nanotubes arrays for application to enzyme biosensors. Biosensors and Bioelectronics, 18(7), 943-951. https://doi.org/10.1016/S0956-5663(02)00209-9 (Original work published 2003)