Human immunoglobulin adsorption investigated by means of quartz crystal microbalance dissipation, atomic force microscopy, surface acoustic wave, and surface plasmon resonance techniques

Zhou, C;Friedt, JM;Angelova, A;Choi, KH;Borghs, G;et.al.
(2004) Langmuir : the A C S journal of surfaces and colloids — Vol. 20, n° 14, p. 5870-5878 (2004)

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Authors
  • Zhou, C
    Author
  • Friedt, JM
    Author
  • Angelova, A
    Author
  • Choi, KH
    Author
  • Francis, Laurentorcid-logoUCLouvain
    Author
  • Borghs, G
    Author
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Abstract
Time-resolved adsorption behavior of a human immunoglobin G (hIgG) protein on a hydrophobized gold surface is investigated using multitechniques: quartz crystal microbalance/dissipation (QCM-D) technique; combined surface plasmon resonance (SPR) and Love mode surface acoustic wave (SAW) technique; combined QCM-D and atomic force microscopy (AFM) technique. The adsorbed hIgG forms interfacial structures varying in organization from a submonolayer to a multilayer. An "end-on" IgG orientation in the monolayer film, associated with the surface coverage results, does not corroborate with the effective protein thickness determined from SPR/SAW measurements. This inconsistence is interpreted by a deformation effect induced by conformation change. This conformation change is confirmed by QCM-D measurement. Combined SPR/SAW measurements suggest that the adsorbed protein barely contains water after extended contact with the hydrophobic surface. This limited interfacial hydration also contributed to a continuous conformation change in the adsorbed protein layer. The viscoelastic variation associated with interfacial conformation changes induces about 1.5 times overestimation of the mass uptake in the QCM-D measurements. The merit of combined multitechnique measurements is demonstrated.
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Zhou, C., Friedt, J., Angelova, A., Choi, K., Laureyn, W., Frederix, F., Francis, L., Campitelli, A., Engelborghs, Y., & Borghs, G. (2004). Human immunoglobulin adsorption investigated by means of quartz crystal microbalance dissipation, atomic force microscopy, surface acoustic wave, and surface plasmon resonance techniques. Langmuir : the A C S journal of surfaces and colloids, 20(14), 5870-5878. https://doi.org/10.1021/la036251d (Original work published 2004)