Capacitive biosensing of bacterial cells: Analytical model and numerical simulations

Couniot, Numa;Afzalian, Aryan;Van Overstraeten, Nancy;Francis, Laurent;Flandre, Denis
(2015) Sensors and Actuators B: Chemical : international journal devoted to research and development of physical and chemical transducers — Vol. 211, p. 428-438 (2015)

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Authors
  • Couniot, NumaUCLouvain
    Author
  • Afzalian, AryanUCLouvain
    Author
  • Van Overstraeten, NancyUCLouvain
    Author
  • Francis, Laurentorcid-logoUCLouvain
    Author
  • Flandre, DenisUCLouvain
    Author
Abstract
Impedimetric biosensors with a passivation layer, also called capacitive biosensors, have recently shown great promise towards sensitive, selective and rapid detection of pathogen bacterial cells. However, few studies focus on their modeling, yet critical for the optimization of their sensitivity. To address this issue, we propose a comprehensive framework by developing analytical models and 2D numerical simulations of passivated interdigitated microelectrodes (IDEs) with adherent bacterial cells in electrolyte. While models provide a qualitative and semi-quantitative analysis of the AC impedance spectroscopy based on the system cutoff frequencies, Finite Element Method (FEM) simulations based on Poisson-Nernst-Planck equations enable accurate quantification of the sensitivity to bacteria versus the applied frequency thanks to modeling of complex phenomena such as ion transport, surface and space charges, multi-shell bacterial dielectric properties and sensor topology. These numerical simulations are assessed by experimental results and compared to analytical models.
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Couniot, N., Afzalian, A., Van Overstraeten, N., Francis, L., & Flandre, D. (2015). Capacitive biosensing of bacterial cells: Analytical model and numerical simulations. Sensors and Actuators B: Chemical : international journal devoted to research and development of physical and chemical transducers, 211, 428-438. https://doi.org/10.1016/j.snb.2015.01.108 (Original work published 2015)