Assessment of different functionalization methods for grafting a protein to an alumina-covered biosensor.

Van Overstraeten, Nancy;Lefèvre, O;Couniot, Numa;Flandre, Denis
(2014) Biofabrication — Vol. 6, n° 3, p. 35007 (2014)

Files

14_05_05_VanOverstraeten_Biofabrication.pdf
  • Closed Access
  • Adobe PDF
  • 2.38 MB
Request a copy
Assessmentofdifferentfunctionalizationmethodsforgraftingaproteintoanalumina-coveredbiosensor.pdf
  • Restricted Access
  • Adobe PDF
  • 2.35 MB
Request a copy

Details

Authors
  • Van Overstraeten, NancyUCLouvain
    Author
  • Lefèvre, O
    Author
  • Couniot, NumaUCLouvain
    Author
  • Flandre, DenisUCLouvain
    Author
Abstract
The specificity of biosensors is typically obtained by surface biofunctionalization, which enables the selective binding of biomolecules. This critical step is sensitive to the nature of materials and to the overall experimental conditions. Here, we provide a comprehensive study of several biofunctionalization methods, including the layer-by-layer technique and both the gas-phase and liquid-phase silanizations, and we propose a new maleimide-based protocol for grafting a protein to a sensor covered by alumina. This method was then validated by making a respiratory syncitial virus-specific biosensor.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Van Overstraeten, N., Lefèvre, O., Couniot, N., & Flandre, D. (2014). Assessment of different functionalization methods for grafting a protein to an alumina-covered biosensor. Biofabrication, 6(3), 35007. https://doi.org/10.1088/1758-5082/6/3/035007 (Original work published 2014)