SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels
(2013) Applied Microbiology and Biotechnology — Vol. 97, n° 22, p. 9811-9824 (2013)
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- Abstract
- In this work, a three-level Salmonella detection system based on a combination of seven SYBR®Green qPCR was developed. This detection system discriminates Salmonella at the genus, species and subspecies levels using a single 96-well plate. The SYBR®Green qPCR assays target the invA, rpoD, iroB and safC genes, as well as the STM0296 locus, putatively coding for a cytoplasmic protein. This study includes the design of primer pairs, in silico and in situ selectivity, sensitivity, repeatability and reproducibility evaluations of the seven SYBR®Green qPCR assays. Each detection level displayed a selectivity of 100 %. This combinatory SYBR®Green qPCR system was also compared with three commercially available Salmonella qPCR detection kits. This comparison highlighted the importance of using a multi-gene detection system to be able to detect every target strain, even those with deletion or mutation of important genes. © 2013 The Author(s).
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Citations
Barbau-Piednoir, E., Bertrand, S., Mahillon, J., Roosens, N. H., & Botteldoorn, N. (2013). SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels. Applied Microbiology and Biotechnology, 97(22), 9811-9824. https://doi.org/10.1007/s00253-013-5234-x (Original work published 2013)