One-day pulsed-field gel electrophoresis protocol for rapid determination of emetic Bacillus cereus isolates

Kaminska, Paulina S.;Fiedoruk, Krzysztof;Jankowska, Dominika;Mahillon, Jacques;Swiecicka, I zabela;et.al.
(2015) Electrophoresis — Vol. 36, n° 7-8, p. 1051-1054 (2015)

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Authors
  • Kaminska, Paulina S.University of Bialystok, Poland
    Author
  • Fiedoruk, KrzysztofUniversity of Bialystok, Poland
    Author
  • Jankowska, DominikaUniversity of Bialystok, Poland
    Author
  • Mahillon, JacquesUCLouvain
    Author
  • Swiecicka, I zabelaUniversity of Bialystok, Poland
    Author
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Abstract
Bacillus cereus, the Gram-positive and spore-forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat stable emetic toxin. Rapid determination of cereulide-positive B. cereus isolates is of highest importance due to consequences of this intoxication for human health and life. Here we present a one-day pulsed-field gel electrophoresis for emetic B. cereus isolates, which allows rapid and efficient determination of their genomic relatedness and helps determining the source of intoxication in case of outbreaks caused by these bacilli.
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Kaminska, P. S., Fiedoruk, K., Jankowska, D., Mahillon, J., Nowosad, K., Drewicka, E., & Swiecicka, I. z. (2015). One-day pulsed-field gel electrophoresis protocol for rapid determination of emetic Bacillus cereus isolates. Electrophoresis, 36(7-8), 1051-1054. https://doi.org/10.1002/elps.201400552 (Original work published 2015)