Detection of Listeria spp. and Listeria monocytogenes in Food and Feed Products
(2014) Listeria monocytogenes: Food Sources, Prevalence and Management Strategies — ISBN: [978-1631170546], 147-166, published
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- Listeria monocytogenes is one of the most important foodborne pathogens because of the high mortality rate of this pathogen infection. Thus, its presence in food along the food chain is intensively controlled. The detection of Listeria in food samples is traditionally performed by culture-based methods using selective enrichment and plating media. The isolated strains should then be confirmed by biochemical characterization. This method, described in the ISO 11290-1, is the gold standard but is time consuming and may not be suitable to test food with short shelf life or in the case of outbreaks. Faster alternative methods based on different technologies have been developed and validated, they are antibodies-based (ELISA, ELFA) or acid nucleic based (RNA or DNA hybridization, PCR, RT-PCR, qPCR) technologies. In this chapter, the standard method for detecting L. monocytogenes in food and feed products as well as the different alternative technologies currently available are described in detail. The advantages and drawbacks of each technology are presented as well as a time scale comparison between the different technologies.
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Barbau-Piednoir, E., Mahillon, J., Roosen, N. H., & Botteldoorn, N. (2014). Detection of Listeria spp. and Listeria monocytogenes in Food and Feed Products. In Editors: Edmund C. Hambrick (ed.), Listeria monocytogenes: Food Sources, Prevalence and Management Strategies (pp. 147-166). Nova Science Pub. https://hdl.handle.net/2078.5/32424