Expansion and Retroviral Transduction of Primary Murine T Cells for CAR T-Cell Therapy
(2023) (2023)
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- Abstract
- The development of chimeric antigen receptor (CAR) T cells has been a revolutionary technology for the treatment of relapsed and refractory leukemias and lymphomas. The synthetic CAR molecule redirects T cell function toward tumor surface-expressed antigens through a single-chain variable fragment (scFv) fused to CD3z and intracellular costimulatory domains. Here, we describe a protocol for the generation of CAR T cells using primary mouse T cells and a gammaretroviral vector encoding a CAR transgene. This protocol outlines several transduction and expansion methods based on the use of two transduction enhancers, RetroNectin ® and Vectofusin ®-1, and cell culture systems such as conventional plates or G-Rex ® devices.
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Citations
Loos, P., Short, L., Savage, G., & Evgin, L. (2023). Expansion and Retroviral Transduction of Primary Murine T Cells for CAR T-Cell Therapy. Published. https://doi.org/10.1007/978-1-0716-3593-3_4 (Original work published 2023)