Layer-by-Layer Self-Assembled Nanotubes for Drug Delivery Applications
(2014) IACIS 2015 — Location: Mainz (Germany) (24.May.2015)
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- Abstract
- Recent advances in medicine and nanotechnology have inspired numerous researches in which nano-engineering is used for the development of optimal drug nanocarrier. These nanovehicles should ideally be capable of protecting the drug from any premature degradation until its arrival to the targeted cells, where the cargo should be released after a fixed time or in response to a specific stimulus. Up to now, most efforts in this field have been focused on creating spherical nanocarriers such as liposomes, capsules, or polymersomes [1]. However, anisotropic structures such as nanotubes are currently attracting considerable attention because they offer some promising advantages over isotropic structures [2,3]. The size of nanotubes can be manipulated in two dimensions, giving the opportunity to construct nanotubes with different aspect ratios. They possess higher surface area compared to nanospheres of identical radius, resulting in more functional groups (antifouling, targeting…) assembled on their surface. Elaborated structures can be achieved by separately and selectively functionalizing the inner and outer surfaces of the nanotubes. The degree of sophistication can still be increased by assembling functional groups on the lateral and basal surfaces of nanotubes. In this work, synthetic and protein-based nanotubes are prepared by combining layer-by-layer (LbL) assembly of polyelectrolytes with hard templating. In one typical system, green-fluorescent tagged protein (ovalbumin tagged with Alexa fluor 488® dye) is used as polyanion and combined with a polyamino acid (PLA) as polycation in order to build multilayered nanotubular structures within the pores of a nanoporous polycarbonate (PC) membrane. Nanotubes are then released from the template by dissolving the membrane and are collected by filtration in presence of a water-soluble adjuvant. We proved that the nature of adjuvant plays an important role in collection process. Collected nanotubes are then dispersed in aqueous solution and are put in contact with dendritic cells (DC2.4) to study cell/ nanotubes interaction. Our first results show that nanotubes have successfully entered the cytoplasm of the cells. Complementary systematic studies are underway to study cell transfection depending on geometry and chemistry of the nanotubes.
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Saghazadeh, S., De Geest, B., Jonas, A., & Demoustier, S. (2014). Layer-by-Layer Self-Assembled Nanotubes for Drug Delivery Applications. IACIS 2015, Mainz (Germany). https://hdl.handle.net/2078.5/270239