Self-Assembly of Protamine Biomacromolecule on Halloysite Nanotubes for Immobilization of Superoxide Dismutase Enzyme

Katana, Bojana;Rouster, Paul;Varga, Gábor;Muráth, Szabolcs;Szilagyi, Istvan;et.al.
(2020) ACS Applied Bio Materials — Vol. 3, n° 1, p. 522-530 (2019)

Files

BKatana-ACSAppliedBioMaterials.pdf
  • Open Access
  • Adobe PDF
  • 1.5 MB
Download

Details

Authors
  • Katana, BojanaMTA-SZTE Lendület Biocolloids Research Group, University of Szeged, H-6720 Szeged, Hungary
    Author
  • Rouster, PaulUCLouvain
    Author
  • Varga, GáborDepartment of Organic Chemistry, University of Szeged, H-6720 Szeged, Hungary
    Author
  • Muráth, SzabolcsMTA-SZTE Lendület Biocolloids Research Group, University of Szeged, H-6720 Szeged, Hungary; Interdisciplinary Excellence Center, Department of Physical Chemistry and Materials Science, University of Szeged, H-6720 Szeged, Hungary
    Author
  • Glinel, Karineorcid-logoUCLouvain
    Author
  • Jonas, Alainorcid-logoUCLouvain
    Author
  • Szilagyi, Istvanorcid-logoMTA-SZTE Lendület Biocolloids Research Group, University of Szeged, H-6720 Szeged, Hungary; Interdisciplinary Excellence Center, Department of Physical Chemistry and Materials Science, University of Szeged, H-6720 Szeged, Hungary
    Author
Show more
Abstract
An antioxidant material composed of halloysite nanotubes (HNTs), protamine sulfate polyelectrolyte (PSP) and superoxide dismutase (SOD) enzyme was prepared by self-assembly of the PSP and SOD biomacromolecules on the nanoparticulate support. The structural, colloidal and biocatalytic features were assessed. Adsorption of PSP on the oppositely charged HNT surface at appropriate loadings gave rise to charge neutralization and overcharging, which resulted in unstable and stable dispersions, respectively. The formation of a saturated PSP layer on the HNT led to the development of positive surface charge and to remarkable resistance against salt-induced aggregation making the obtained HNT-PSP hybrid suitable for immobilization of negatively charged SOD. No enzyme leakage was observed from the HNT-PSP-SOD composite indicating sufficient structural stability of this material due to electrostatic, hydrophobic and hydrogen bonding interactions taking place between the particles and the biomacromolecules. Enzymatic assays revealed that SOD kept its functional integrity upon immobilization and showed high activity in superoxide radical dismutation. In this way, stable antioxidant bionanocomposite dispersions were obtained, which can be used as antioxidants in heterogeneous samples.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Katana, B., Rouster, P., Varga, G., Muráth, S., Glinel, K., Jonas, A., & Szilagyi, I. (2020). Self-Assembly of Protamine Biomacromolecule on Halloysite Nanotubes for Immobilization of Superoxide Dismutase Enzyme. ACS Applied Bio Materials, 3(1), 522-530. https://doi.org/10.1021/acsabm.9b00953 (Original work published 2019)