Exploring xylem endophytes interplay with Xylella fastidiosa or Brenneria salicis
(2024) 75th International Symposium on Crop Protection — Location: Ghent, Belgium (21.May.2024)
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- While Xylella fastidiosa and Brenneria salicis typically induce host decline, asymptomatic cases exist and infected plants sometimes show no symptoms at all. This duality could be explain by recent studies showing interactions between pathogenic bacteria and the endophytic xylem microbiota. Although endophytes significantly contribute to plant health, questions persist about the underlying protective mechanisms. This project aims at assessing how endophytes interfere with phytopathogenic bacteria colonizing the specific xylem niche and if those interactions could result in mitigating symptoms development. Over 350 bacterial endophytes were isolated from xylem sap, revealing potential biocontrol agents. Based on simple confrontation assays, interactions with X. fastidiosa KLN59.3 and B. salicis LMG2698 were investigated in vitro. Endophytes showing inhibition activity against one or both of the bacteria are then tested for siderophore, indole related compound production, and also for phosphate solubilization, swimming or swarming motility and biofilm formation. Based on the confrontation assay and those further tests, eleven promising strains from (B. amyloliquefaciens, B. haynesii, B. pseudomycoides, B. subtilis, B. velzensis) and Pseudomonas (P. coleopterorum, P. graminis) genera, expressing mCherry fluorescent protein, are examined for growth, propagation, and interaction in xylem vessels upon co-inoculation with phytopathogenic bacteria on Salicaceae and Nicotiana tabacum as test species.
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Pesenti, L., & Bragard, C. (2024). Exploring xylem endophytes interplay with Xylella fastidiosa or Brenneria salicis. 75th International Symposium on Crop Protection, Ghent, Belgium. https://hdl.handle.net/2078.5/269714