Interplay of xylem endophytes - Unravelling their interactions with Xylella fastidiosa and Brenneria salicis in Salix and Populus

Pesenti, Lena;Bragard, Claude
(2024) International Conference on Plant Pathogenic Bacteria and Biocontrol — Location: Blacksburg, Virginia, USA (7.July.2024)

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Abstract
If Xylella fastidiosa and Brenneria salicis, two xylem-specialist phytopathogenic bacteria, often cause the complete decline of their host, infected plants sometimes show no symptoms at all. No factor has yet been identified to explain this dual symptomatology. However, recent studies show interactions between pathogenic bacteria and the endophytic xylem microbiota. Endophytes contribute significantly to plant health. If this is widely accepted, many questions remain about the mechanisms underlying such protection. This project aims at assessing how endophytes interfere with phytopathogenic bacteria colonizing the specific xylem niche. Our study is based on the current knowledge of the interaction between Salicaceae plant-host and both bacteria investigated. After an endophyte screening phase by isolation, interactions with X. fastidiosa KLN59.3 and B. salicis LMG2698 were investigated based on simple confrontation assays. Endophytes showing inhibition activity against one or both of the bacteria are then tested for siderophore, indole related compound production, and also for phosphate solubilization, swimming or swarming motility and biofilm formation. Endophytic strains of interest, mainly from Bacillus and Pseudomonas genera, are transformed to express a mCherry fluorescent protein to see their growth, propagation and interaction in the xylem vessels after co-inoculation with phytopathogenic bacteria.
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Pesenti, L., & Bragard, C. (2024). Interplay of xylem endophytes - Unravelling their interactions with Xylella fastidiosa and Brenneria salicis in Salix and Populus. International Conference on Plant Pathogenic Bacteria and Biocontrol, Blacksburg, Virginia, USA. https://hdl.handle.net/2078.5/269690