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Akkermansiamuciniphilamodulatesintestinalmucuscompositiontocounteracthigh-fatdiet-inducedobesityinmice.pdf
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This study investigates whether live Muc supplementation can counteract obesity and metabolic dysfunctions induced by a high-fat diet (HFD) by modulating intestinal mucus production, secretion and composition. C57BL/6J mice were fed an HFD with or without live Muc (2 × 10 CFU per day) supplementation or a control diet for 6 weeks. Body weight, fat mass gain and metabolic markers were measured. Intestinal mucus characteristics were assessed via gene expression analysis of mucins and analysed mucin glycosylation by tandem mass spectrometry (MS/MS). Mice receiving live Muc exhibited reduced body weight gain and fat mass accumulation compared to HFD controls, without changes in muscle mass. improved gut barrier integrity by increasing antimicrobial peptide expression in the jejunum and in the colon of HFD-fed mice. Furthermore, live Muc influenced markers of goblet cell differentiation and restored the expression of mucin markers altered by HFD. Specifically, live Muc counteracted HFD-induced mucin 3 (Muc3) expression depletion in the colon. Although the overall mucus thickness was not affected by live Muc, the bacteria significantly modulated mucin glycans composition. Live Muc did not change the gut microbiota composition. These findings highlight the protective effects of live Muc against diet-induced metabolic dysfunctions by modulating adiposity, mucus layer composition, and glycan profiles. This reinforces its potential as a therapeutic strategy for metabolic disorders associated with gut microbiota alterations.
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Paone, P., Petitfils, C., Puel, A., Latousakis, D., de Vos, W. M., Delzenne, N. M., Juge, N., Van Hul, M., & Cani, P. D. (2026). . Gut microbes, 18(1), 2612580. https://doi.org/10.1080/19490976.2025.2612580 (Original work published 2026)