Improving the profiling of wheat bacterial and fungal endophytic communities—a PCR clamping approach
(2025) Frontiers in Microbiology — Vol. 16, n° 13.3389, p. 1-14 (2025)
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- Abstract
- Plant-associated endophytic microbial communities are an important source of biological diversity. To study them, efficient, robust, and standardized characterization methods are necessary. These communities are usually profiled using amplicon high-throughput sequencing (metabarcoding), but the large amount of host DNA often leads to substantial co-amplification of organellar sequences, thereby hampering accurate characterization. A promising solution is the use of PCR clamps, modified oligomers that block non-target DNA amplification. However, no practical guidelines are currently available to support their development, and no sets of clamps enabling comprehensive characterization of endophytic bacterial and fungal communities associated with wheat (Triticum aestivum ssp. aestivum) have been reported.
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Citations
Dubois, B., Delitte, M., Bragard, C., Legrève, A., Chandelier, A., & Debode, F. (2025). Improving the profiling of wheat bacterial and fungal endophytic communities—a PCR clamping approach. Frontiers in Microbiology, 16(13.3389), 1-14. https://doi.org/10.3389/fmicb.2025.1690976 (Original work published 2025)