Sequences analysis of Belgian BNYVV and development of a simultaneous detection of soil-borne sugar beet viruses by RT-PCR
(2003) International Working Group on Plant Viruses with Fungal Vectors — Location: Zurich, Suisse
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- Sugar beets and soils suspected of being infected by rhizomania have been collected in more than one hundred locations in Belgium. Each sample was analysed by DAS-ELISA and RT-PCR targeting BNYVV RNA-5 and the readthrough protein of RNA-2. BNYVV was detected by RT-PCR in more than 40% of the fields targeted. None of these isolates showed the presence of RNA-5. BNYVV RNA-3 of 27 Belgian isolates was sequenced from nt. 446 to nt.1000. Based on these results, B type was found mainly in the eastern areas of Belgium while A type was detected in the west. The identity at the nucleotide level between the isolates was 100% for the B type, 98.9% for the type A and 96.0% between the two types. Nine of the 11 mutations observed between the two types lead to amino acid changes. Amino acids of p25 protein of RNA-3 shows 100%, 97.2% and 94.6% of identity for the B type, the A type and between the two respectively. A multiplex RT-PCR detecting BNYVV, Polymyxa betae and Beet soilborne virus (BSBV) and Beet virus Q (BVQ) was developed to facilitate their detection. The technique revealed that the latter two are present in more than 80% of the fields tested as well as in Asian and European soils. The exact role of these pomoviruses in the disease of rhizomania is not yet clearly demonstrated though their simultaneous occurrence in sugar beets raises questions.
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Meunier, A., Schmit, J.-F., & Bragard, C. (2003). Sequences analysis of Belgian BNYVV and development of a simultaneous detection of soil-borne sugar beet viruses by RT-PCR. In C.M. Rush and U. Merz (ed.), Proceedings of the Fifth Symposium of the International Working Group on Plant Viruses with Fungal Vectors. American society for sugar beet technologist. https://hdl.handle.net/2078.5/255944