Genetic characterization of Pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombination
(2008) European Journal of Plant Pathology — n° 121, p. 131-146 pp (2008)
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- Abstract
- Over a period of a few years, Pepino mosaic virus (PepMV) has become one of the most important viral diseases in tomato production worldwide. Infection by PepMV can cause a broad range of symptoms on tomato plants, often leading to significant financial loses. At present, five PepMV genotypes (EU,LP, CH2, US1 and US2) have been described, three of which (EU, LP and US2) have been reported in Europe. Thus far, no correlation has been found between different PepMV genotypes and the symptomps expressed in infected plants.In this paper, the genetic diversity of the PepMV population in Belgian greenhouses is studied and related to sympton development in tomato crops. A novel assay based on restriction fragment length polymorphism (RFLP) was developed to descrimate between the different PepMV genotypes. Both RFLP and sequence analysis revealed the occurrence of two genotypes, the EU genotype and the CH2 genotype, within tomato production in Belgium. Whereas no differences were observed in symptom expression between plants infected by one of the two genotypes, co-infection with both genotypes resulted in more severe PepMV symptoms. Furthermore, our study revealed that PepMV recombinants frequently occur in mixed infections under natural conditions. This may possibly result in the generation of viral variants with increased aggressiveness.
- Affiliations
Scientia Terrae Research Institute (Belgium) Scientia Terrae Reserach Institute Scientia Terrae Research Institute (Belgium) Scientia Terrae Research Institute Research Station for Vegetable Production Research Station for Vegetable Production Research Centre Hoogstraten (Belgium) Research Centre Hoogstraten Wageningen University Laboratory of Phytopathology
Citations
Hanssen, I. M. (2008). Genetic characterization of Pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombination. European Journal of Plant Pathology, 121, 131-146 pp. https://hdl.handle.net/2078.5/255903 (Original work published 2008)