The alarm pheromone E-B-Farnesene as biocontrol semiochemical to reduce potato virus y propagation in potato fields

Crutzen, François;Moreau, Virginie;Francis, François;Bragard, Claude
(2014) 19th Triennial Conference of the European Association for Potato Research — Location: Brussels, Belgium (6.July.2014)

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Abstract
The Potato virus Y (PVY) Potyvirus is transmitted to potatoes in a non-persistent manner by aphids. E-B-Farnesene (EBF) is a largely interspecific aphid alarm pheromone which, depending on multiple factors, might have an impact on aphids feeding, winging, moving or escaping behaviors. In addition, predators and parasitoids may also be stimulated by EBF to find aphids. In preliminary field trials, EBF had been shown to impact PVY field transmission by aphids. With the view of developing an innovative biocontrol method for PVY infestations on potatoes, we assessed the impact of EBF on the virus propagation under field trials carried out in 2013. To ensure both virus and vector pressure, PVY was inoculated on 11% of the plants. Aphids (Myzus persicae) were artificially introduced in the plots. Potato leaves were sampled at four collection dates during the growing season and stored at -20°C before RNA extractions. Quantitative RT-PCR was performed to determine the PVY infection rates in the potato plots depending on whether EBF was released or not.
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Crutzen, F., Moreau, V., Francis, F., & Bragard, C. (2014). The alarm pheromone E-B-Farnesene as biocontrol semiochemical to reduce potato virus y propagation in potato fields. 19th Triennial Conference of the European Association for Potato Research, Brussels, Belgium. https://hdl.handle.net/2078.5/255825