First report ofPotato yellow mosaic virusinfectingSolanum americanumin Venezuela
(2016) New Disease Reports — Vol. 34, p. 20 (2016)
Files
NDR_034020.pdf
Restricted Access - Adobe PDF
- 636.01 KB
Details
- Authors
- Abstract
- Potato yellow mosaic virus (PYMV) is one of the most important tomato begomoviruses in the Caribbean basin (Morales et al., 2006). In Venezuela, a recent survey showed that PYMV is the most widespread and prevalent begomovirus in tomato fields (Geraud-Pouey et al., 2016). However, little is known about natural reservoirs of the virus. In 2007 and 2008 PYMV surveys were performed in several Venezuelan tomato fields and weeds in the genus Solanum showing virus-like symptoms were also collected (Fig. 1). As summarised in Table 1, nine leaf samples from solanaceous weeds were tested using PCR with PYMV-specific primers (Geraud-Pouey et al., 2016). PYMV infection was detected in two plants each of Solanum americanum and S. pimpinellifolium. Solanum pimpinellifolium is considered a host of PYMV (Boissot et al., 2008). However, S. americanum has not been found previously to be infected by this begomovirus. Hence, the complete sequence of DNA-A of PYMV isolates from the two S. americanum plants (herafter, Tachira-949 and Lara-1203) were obtained by rolling circle-amplification (RCA) using ϕ29 DNA polymerase (TempliPhi kit, GE Healthcare, Germany). The RCA products were then digested with the endonuclease HindIII and inserted in pBluescript II (SK+) for cloning. The complete sequences of DNA-A of isolates Tachira-949 (2,597 bp) and Lara-1203 (2,596 bp) were deposited in GenBank under Accession Nos. KU665804 and KX389269, respectively. Blast analyses revealed that PYMV isolate (D00940) from Venezuela was the most closely related isolate to the isolates Tachira-949 and Lara-1203 showing 94 and 93% nucleotide identity, respectively. PYMV-like sequences in GenBank were retrieved to perform pairwise and phylogenetic analyses using Mega 6 (Tamura et al., 2013). Isolates Tachira-949 and Lara-1203 shared 96% nucleotide identity and they were most related phylogenetically to PYMV isolates from Central America and Venezuela (Fig. 2). Interestingly, the two PYMV-infected S. americanum plants were found in geographically distant tomato production zones of the country.
- Affiliations
Citations
Romay Gonzalez, G., Chirinos, D. T., Geraud-Pouey, F., Torres, M., & Bragard, C. (2016). First report ofPotato yellow mosaic virusinfectingSolanum americanumin Venezuela. New Disease Reports, 34, 20. https://doi.org/10.5197/j.2044-0588.2016.034.020 (Original work published 2016)