The Complete Genome Sequence of Xanthomonas theicola, the Causal Agent of Canker on Tea Plants, Reveals Novel Secretion Systems in Clade-1 Xanthomonads
(2021) Phytopathology — Vol. 111, n° 4, p. 611-616 (2021)
Files
PHYTO-07-20-0273-SC.pdf
Open Access - Adobe PDF
- 426.54 KB
Details
- Authors
Koebnik, Ralf 
Jacobs, Jonathan M.
- Abstract
- Xanthomonas theicola is the causal agent of bacterial canker on tea plants. There is no complete genome sequence available for X. theicola, a close relative of the species X. translucens and X. hyacinthi, thus limiting basic research for this group of pathogens. Here, we release a high-quality complete genome sequence for the X. theicola type strain, CFBP 4691T. Single-molecule real-time sequencing with a mean coverage of 264× revealed two contigs of 4,744,641 bp (chromosome) and 40,955 bp (plasmid) in size. Genome mining revealed the presence of nonribosomal peptide synthases, two CRISPR systems, the Xps type 2 secretion system, and the Hrp type 3 secretion system. Surprisingly, this strain encodes an additional type 2 secretion system and a novel type 3 secretion system with enigmatic function, hitherto undescribed for xanthomonads. Four type 3 effector genes were found on complete or partial transposons, suggesting a role of transposons in effector gene evolution and spread. This genome sequence fills an important gap to better understand the biology and evolution of the early-branching xanthomonads, also known as clade-1 xanthomonads.
- Affiliations
Citations
Koebnik, R., Burokiene, D., Bragard, C., Chang, C., Saux, M. F.-L., Kölliker, R., Lang, J. M., Leach, J. E., Luna, E. K., Portier, P., Sagia, A., Ziegle, J., Cohen, S. P., & Jacobs, J. M. (2021). The Complete Genome Sequence of Xanthomonas theicola, the Causal Agent of Canker on Tea Plants, Reveals Novel Secretion Systems in Clade-1 Xanthomonads. Phytopathology, 111(4), 611-616. https://doi.org/10.1094/phyto-07-20-0273-sc (Original work published 2021)