Nicotiana tabacum contains two alpha1,3-fucosyltransferase types, one of which is able to catalyze core fucosylation of high-mannose N-glycans
(2025) Glycobiology — Vol. 35, n° 6, p. 1-12 (2025)
Files
NavarreetalversionaccepéeGlycobiology2025.pdf
Open Access - Adobe PDF
- 8.76 MB
Details
- Authors
- Abstract
- N-glycosylationisacriticalqualityattributetoconsiderwhenexpressingrecombinantglycoproteinsineukaryoticcellsincludingplantcells.N-acetylglucosaminyltransferaseI(GnTI)initiatescomplexN-glycanmaturationintheGolgiapparatusbytransferringasingleN-acetylglucosamine(GlcNAc)residueonthealpha1,3-armofaMan5N-glycanacceptor.ThisstepisrequiredfortheprocessingofhighmannoseintohybridandcomplexN-glycans.Therefore,ArabidopsismutantslackingGnTIactivitydisplayaccumulationofMan5N-glycansinsteadofcomplexN-glycansanddonotsynthesiseN-glycanscontainingcorealpha1,3-fucoseresidue.Incontrast,GnTIknockoutcelllineofNicotianatabacumBY-2stilldisplaysalittlecorealpha1,3-fucosesignalonwesternblotting.Here,weshowthatN.tabacumcontainstwoalpha1,3-fucosyltransferasetypes,oneofwhichisabletotransferacorealpha1,3-fucoseonaMan5substratewhennoMan5GnsubstrateisavailablesuchasinBY-2GnTIknock-outcelllines.
- Affiliations
Citations
Navarre, C., BAILLY, N., Balieu, J., Perruchon, O., Herman, X., Mercier, A., Courtoy, A., Lerouge, P., Bardor, M., & Chaumont, F. (2025). Nicotiana tabacum contains two alpha1,3-fucosyltransferase types, one of which is able to catalyze core fucosylation of high-mannose N-glycans. Glycobiology, 35(6), 1-12. https://doi.org/10.1093/glycob/cwaf024 (Original work published 2025)