Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese
(2020) PLoS One — Vol. 15, n° 4, p. e0231634 [1-14] (2020)
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Scarcello, Eleonora 
Lison, Dominique
- Abstract
- The WST-1 assay is the most common test to assess the in vitro cytotoxicity of chemicals. Tetrazolium-based assays can, however, be affected by the interference of tested chemicals,including carbon nanotubes or Mg particles. Here, we report a new interference of Mn materials with the WST-1 assay. Endothelial cells exposed to Mn particles (Mn alone or Fe-Mn alloy from 50 to 1600 μg/ml) were severely damaged according to the WST-1 assay, but not the ATP content assay. Subsequent experiments revealed that Mn particles interfere with the reduction of the tetrazolium salt to formazan. Therefore, the WST-1 assay is not suitable to evaluate the in vitro cytotoxicity of Mn-containing materials, and luminescencebased assays such as CellTiter-Glo® appear more appropriate.
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Citations
Scarcello, E., Lambremont, A., Vanbever, R., Jacques, P., & Lison, D. (2020). Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese. PLoS One, 15(4), e0231634 [1-14]. https://doi.org/10.1371/journal.pone.0231634 (Original work published 2020)