Possible crosstalk between the Arabidopsis TSPO-related protein and the transcription factor WRINKLED1
(2024) Biochimie : an international journal of biochemistry and molecular biology — Vol. 224, p. 62-70 (2024)
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- Authors
Bhati, Kaushal Kumar 
Luong, Ai-My
- Abstract
- This study uncovers a regulatory interplay between WRINKLED1 (WRI1), a master transcription factor for glycolysis and lipid biosynthesis, and Translocator Protein (TSPO) expression in Arabidopsis thaliana seeds. We identified potential WRI1-responsive elements upstream of AtTSPO through bioinformatics, suggesting WRI1's involvement in regulating TSPO expression. Our analyses showed a significant reduction in AtTSPO levels in wri1 mutant seeds compared to wild type, establishing a functional link between WRI1 and TSPO. This connection extends to the coordination of seed development and lipid metabolism, with both WRI1 and AtTSPO levels decreasing post-imbibition, indicating their roles in seed physiology. Further investigations into TSPO's impact on fatty acid synthesis revealed that TSPO misexpression alters WRI1's post-translational modifications and significantly enhances seed oil content. Additionally, we noted a decrease in key reserve proteins, including 12 S globulin and oleosin 1, in seeds with TSPO misexpression, suggesting a novel energy storage strategy in these lines. Our findings reveal a sophisticated network involving WRI1 and AtTSPO, highlighting their crucial contributions to seed development, lipid metabolism, and the modulation of energy storage mechanisms in Arabidopsis.
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Citations
Bhati, K. K., Luong, A.-M., Dittrich-Domergue, F., D’Andrea, S., Moreau, P., & Batoko, H. (2024). Possible crosstalk between the Arabidopsis TSPO-related protein and the transcription factor WRINKLED1. Biochimie : an international journal of biochemistry and molecular biology, 224, 62-70. https://doi.org/10.1016/j.biochi.2024.05.011 (Original work published 2024)