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Protocol for the preclinical evaluation of gut barrier function and immune interaction in an HT-29/PBMC co-culture model.

Astre, Gwendoline;Créchet, Laurianne;Pomié, Nicolas;Pereira, Ophélie;Abot, Anne;et.al.
(2024) STAR protocols — Vol. 5, n° 4, p. 103416 [1-14] (2024)

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Authors
  • Astre, Gwendoline
    Author
  • Créchet, Laurianne
    Author
  • Pomié, Nicolas
    Author
  • Pereira, Ophélie
    Author
  • Cani, Patriceorcid-logoUCLouvain
    Author
  • Knauf, ClaudeUCLouvain
    Author
  • Abot, Anne
    Author
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Abstract
Here, we present a protocol for the pre-clinical evaluation of gut barrier function and immune interaction in an HT-29/PBMC co-culture model. We describe steps for culturing HT-29 cells and peripheral blood mononuclear cells (PBMCs), assembling the co-culture model, and testing cell viability. We then detail procedures for performing efficacy tests through stress challenges and barrier permeability assays. For complete details on the use and execution of this protocol, please refer to Abot et al.
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Astre, G., Créchet, L., Pomié, N., Pereira, O., Cani, P., Knauf, C., & Abot, A. (2024). Protocol for the preclinical evaluation of gut barrier function and immune interaction in an HT-29/PBMC co-culture model. STAR protocols, 5(4), 103416 [1-14]. https://doi.org/10.1016/j.xpro.2024.103416 (Original work published 2024)