Ephrin-B1 Controls the Columnar Distribution of Cortical Pyramidal Neurons by Restricting Their Tangential Migration
(2013) Neuron — Vol. 79, n° 6, p. 1123-1135 (2013)
Files
EphrinB1controlsthecolumnardistribution.pdf
Restricted Access - Adobe PDF
- 4.28 MB
Details
- Authors
- Abstract
- Neurons of the cerebral cortex are organized in layers and columns. Unlike laminar patterning, the mechanisms underlying columnar organization remain largely unexplored. Here, we show that ephrin-B1 plays a key role in this process through the control of nonradial steps of migration of pyramidal neurons. In vivo gain of function of ephrin-B1 resulted in a reduction of tangential motility of pyramidal neurons, leading to abnormal neuronal clustering. Conversely, following genetic disruption of ephrin-B1, cortical neurons displayed a wider lateral dispersion, resulting in enlarged ontogenic columns. Dynamic analyses revealed that ephrin-B1 controls the lateral spread of pyramidal neurons by limiting neurite extension and tangential migration during the multipolar phase. Furthermore, we identified P-Rex1, a guanine-exchange factor for Rac3, as a downstream ephrin-B1 effector required to control migration during the multipolar phase. Our results demonstrate that ephrin-B1 inhibits nonradial migration of pyramidal neurons, thereby controlling the pattern of cortical columns.
- Affiliations
Citations
Dimidschstein, J., Passante, L., Dufour, A., van den Ameele, J., Tiberi, L., Hrechdakian, T., Adams, R., Klein, R., Lie, D., Jossin, Y., & Vanderhaeghen, P. (2013). Ephrin-B1 Controls the Columnar Distribution of Cortical Pyramidal Neurons by Restricting Their Tangential Migration. Neuron, 79(6), 1123-1135. https://doi.org/10.1016/j.neuron.2013.07.015 (Original work published 2013)