This protocol describes a robust method to obtain transgenic Nicotiana tabacum BY-2 cells that produce glycoproteins of interest via Agrobacterium tumefaciens transformation. Compared to biolistics-based transformation, this procedure requires only standard laboratory equipment.
Navarre, C., & Chaumont, F. (2022). Production of Recombinant Glycoproteins in Nicotiana tabacum BY-2 Suspension Cells. In Stefan Schillberg, Holger Spiegel (ed.), Recombinant Proteins in Plants : Methods in Molecular Biology (p. p. 81-88). https://doi.org/10.1007/978-1-0716-2241-4_5