Non-invasive isotope-based hydrodynamic imaging in plant roots at cellular resolution

Couvreur, Valentin;Pascut, Flavius C;Dietrich, D;Leftley, N.;Draye, Xavier;et.al.
(2022) European Geophysical Union (EGU) General Assembly 2022 — Location: Vienna (23.May.2022)

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Authors
  • Couvreur, Valentinorcid-logoUCLouvain
    Author
  • Pascut, Flavius CFaculty of Engineering, University of Nottingham, Nottingham, UK
    Author
  • Dietrich, DSchool of Biological Sciences, University of Bristol, Bristol, UK
    Author
  • Leftley, N.School of Biosciences, University of Nottingham, Sutton Bonington, UK
    Author
  • Draye, Xavierorcid-logoUCLouvain
    Author
  • et. al.
Abstract
A key impediment to studying water-related mechanisms in plants is the inability to noninvasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling, can achieve this goal - monitoring deuterated water fluxes within living root tissues at cell- and sub-second-scale resolutions. Raman imaging of water-transporting xylem vessels in Arabidopsis thaliana mutant roots reveals faster xylem water transport in endodermal diffusion barrier mutants. Furthermore, transverse line scans across the root suggest water transported via the root xylem does not re-enter outer root tissues nor the surrounding soil when en-route to shoot tissues if endodermal diffusion barriers are intact, thereby separating ‘two water worlds’ inside roots.
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Couvreur, V., Pascut, F. C., Dietrich, D., Leftley, N., Draye, X., & et al. (2022). Non-invasive isotope-based hydrodynamic imaging in plant roots at cellular resolution. European Geophysical Union (EGU) General Assembly 2022, Vienna. https://hdl.handle.net/2078.5/222361