Deciphering the conjugation system of pXO16 from Bacillus thuringiensis serovar israelensis
(2017) ASM Microbe 2017 — Location: New Orleans, United States of America (1.June.2017)
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- The entomopathogenic Bacillus thuringiensis serovar israelensis displays peculiar conjugative transfer capabilities, accounted for by the very large conjugative plasmid pXO16 (350 kb). The efficient and fast conjugative transfers are accompanied by a spectacular macroscopic aggregation of bacterial partners in liquid matings. Moreover, pXO16 is capable of effective mobilization and retro-transfer of both mobilizable and “non-mobilizable” plasmids. Analysis of pXO16 sequence did not reveal any obvious type IV secretion system (T4SS) homologs, which suggests the presence of an unusual conjugative system in B. thuringiensis. However, the presence in pXO16 of an FtsK/SpOIIIE CDS homolog could be reminiscent of either the Streptomyces spp. TraB-mediated dsDNA transfer system or the Clostridium perfringens tcp conjugation locus. In this work, we aimed to identify the genes responsible for pXO16 transfer mechanism, to define the role of the FtsK/SpOIIIE homolog and to gain better insights into pXO16 unique aggregation system.
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Makart, L., Commans, F., Gillis, A., & Mahillon, J. (2017). Deciphering the conjugation system of pXO16 from Bacillus thuringiensis serovar israelensis. ASM Microbe 2017, New Orleans, United States of America. https://hdl.handle.net/2078.5/220850