Genetic diversity and symbiotic effectiveness of indigenous root nodulating bacteria associated with soybean in Benin (West Africa)
(2023) Conference: World Soybean Research Conference 11 — Location: Vienna (18.June.2023)
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- Nitrogen is one of the deficient nutrients for soybean [Glycine max (L.) Merrill] cropping and can be fully supplied by the biological nitrogen fixation process. Assessment of soybean indigenous rhizobia is very important for identifying strains potentially useful as bio-fertilizer. This study was carried out to assess the genetic diversity, symbiotic effectiveness (SE) and tolerance to abiotic stresses of soybean rhizobia strains from three agroecological zones in Benin. Isolation was performed following standard procedures for Rhizobium study. Symbiotic effectiveness of strains was assessed by inoculating them to soybean seedling in greenhouse and ability of nineteen nodulated strains to promote tolerance to pH, salinity and temperature was tested on specific medium. Nodule number (NN), shoot, root and nodule dry weight (SDW, RDW and NDW respectively), shoot nitrogen content (SNC) and percent of nitrogen derived from the atmosphere (%Nfda) were recorded. Most of the strains were found to be sensitive to extreme pH (3 and 11), high temperature (45°C) and elevated salt concentration (3%), with the presence of some outliers. Nodulation on soybean roots was achieved in 58% of isolated strains. The highest and lowest NN were recorded for the strains BANIL1b and TCHBA2b (66 and 13 nodules plant-1 respectively). Interestingly, the strains varied greatly for their SE relative to the un-inoculated N-fertilized control, with values ranging from 39.95 to 109.09%
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Sinhouenon, B., Touvihoudji, Lienard, C., Nourou Soulemane Yorou, & Bertin, P. (2023). Genetic diversity and symbiotic effectiveness of indigenous root nodulating bacteria associated with soybean in Benin (West Africa). Soybean Research for Sustainable Development, p. https://hdl.handle.net/2078.5/220020