Loss of function mutations at the tomato SSI2 locus impair plant growth and development by altering the fatty acid desaturation pathway
(2024) Plant Biology — Vol. 26, n° 1, p. 106-116 (2023)
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Quevedo‐Colmena, A. S. 
Ortiz‐Atienza, A. Lozano, R.
- Abstract
- The stearoyl-ACP desaturase (SACPD) is a key enzyme in the regulation of saturated to unsaturated fatty acid ratio, playing a crucial role in regulating membrane stability and fluidity, as well as photosynthesis efficiency, which makes it an important research focus in crop species. • This study reports the characterization and molecular cloning of pale dwarf (pad), a new tomato (Solanum lycopersicum L.) T-DNA recessive mutant, which exhibits a dwarf and chlorotic phenotype. Functional studies of the T-DNA tagged gene were conducted, including phylogenetic analysis, expression and metabolomic analyses, and generation of CRISPR/Cas9 knockout lines.
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Quevedo‐Colmena, A. S., Ortiz‐Atienza, A., Jáquez‐Gutiérrez, M., Quinet, M., Atarés, A., Yuste‐Lisbona, F. J., Moreno, V., Angosto, T., & Lozano, R. (2024). Loss of function mutations at the tomato SSI2 locus impair plant growth and development by altering the fatty acid desaturation pathway. Plant Biology, 26(1), 106-116. https://doi.org/10.1111/plb.13591 (Original work published 2023)