Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design.

Ramírez-Cavazos, Leticia I;Junghanns, Charles;Nair, Rakesh;Cárdenas-Chávez, Diana L;Parra, Roberto;et.al.
(2014) Zhejiang University. Journal (Science B) : international biomedicine & biotechnology journal — Vol. 15, n° 4, p. 343-352 (2014)

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Authors
  • Ramírez-Cavazos, Leticia I
    Author
  • Junghanns, Charles
    Author
  • Nair, Rakesh
    Author
  • Cárdenas-Chávez, Diana L
    Author
  • Agathos, Spiros N.orcid-logoUCLouvain
    Author
  • Parra, Roberto
    Author
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Abstract
The production of thermostable laccases from a native strain of the white-rot fungus Pycnoporus sanguineus isolated in Mexico was enhanced by testing different media and a combination of inducers including copper sulfate (CuSO4). The best conditions obtained from screening experiments in shaken flasks using tomato juice, CuSO4, and soybean oil were integrated in an experimental design. Enhanced levels of tomato juice as the medium, CuSO4 and soybean oil as inducers (36.8% (v/v), 3 mmol/L, and 1% (v/v), respectively) were determined for 10 L stirred tank bioreactor runs. This combination resulted in laccase titer of 143000 IU/L (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), pH 3.0), which represents the highest activity so far reported for P. sanguineus in a 10-L fermentor. Other interesting media resulting from the screening included glucose-bactopeptone which increased laccase activity up to 20000 IU/L, whereas the inducers Acid Blue 62 and Reactive Blue 19 enhanced enzyme production in this medium 10 times. Based on a partial characterization, the laccases of this strain are especially promising in terms of thermostability (half-life of 6.1 h at 60 °C) and activity titers.
Affiliations
  • Institution iconUCLouvainSST/ELI/ELIM - Applied Microbiology
  • Technologico de MonterreyCentro del Agua para America y el Caribe
  • Helmholtz Centre for Environmental Research - UFZBioremediation
  • Universidad Autonoma de Nuevo LeonLaboratorio de Enzimologia - Facultad de Ciencias Biologicas

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Ramírez-Cavazos, L. I., Junghanns, C., Nair, R., Cárdenas-Chávez, D. L., Hernández-Luna, C., Agathos, S. N., & Parra, R. (2014). Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design. Zhejiang University. Journal (Science B) : international biomedicine & biotechnology journal, 15(4), 343-352. https://doi.org/10.1631/jzus.B1300246 (Original work published 2014)