Bone marrow mononuclear cell infusion into the liver is associated with liver macrophage expansion in patients with decompendated alcoholic liver disease (ALD)

Lanthier, Nicolas;Rubbia-Brandt, Laura;Chalandon, Yves;Kindler, Vincent;Spahr, Laurent;et.al.
(2012) Annual Meeting of the Swiss Society of Gastroenterology — Location: Interlaken (20.September.2012)

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Authors
  • Lanthier, Nicolasorcid-logoUCLouvain
    Author
  • Rubbia-Brandt, LauraHôpitaux Universitaires de Genève
    Author
  • Chalandon, YvesHôpitaux Universitaires de Genève
    Collaborator
  • Kindler, VincentHôpitaux Universitaires de Genève
    Collaborator
  • Spahr, LaurentHôpitaux Universitaires de Genève
    Author
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Abstract
Background In decompensated ALD, autologous transplantation of bone marrow mononuclear cells doesn’t improve liver function at 3 months as compared to standard of care (Hepatology 2011, Abstract). We aim to analyze changes in hepatic progenitor cell (HPC) and macrophage compartments associated with this strategy. Methods: We examined liver biopsies obtained at baseline and at 4 weeks of follow-up in 58 patients. Both HPC and macrophage compartments were analyzed using immunohistochemistry for CK7 and CD68 and morphometric analysis. Results Immunohistochemical staining for CK7 correlated with liver failure as assessed by the MELD score both at baseline (r=0.33; p<0.05) and at 4-week biopsies (r=0,61; p<0,01), in line with recently published data (Sancho-Bru, Hepatology 2012). Autologous transplantation procedure was not associated with changes in CK7 positive cells at 4-week follow-up biopsy. Table: (CD68 expressed in % of surface area) Treatment Group Baseline CD68 (%) 4-week CD68 (%) P value Bone marrow 3.7 5.4 0.01 Standard of care 4.2 4.6 NS Further analyses of gene expression patterns are planned in order to determine whether those changes in the macrophage compartment are associated modifications in inflammatory or regenerative pathways. Conclusion Total liver cell CK7 positivity correlates with the degree of liver failure in patients with ALD. Autologous mononuclear bone marrow cell infusion doesn’t increase the number of HPC as compared to standard of care alone, but is associated with an expansion of the macrophage compartment. This is of particular interest, knowing the role of the micro-environment in HPC polarization.
Affiliations
  • Hôpitaux Universitaires de GenèveService de Gastro-entérologie et Hépatologie

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Lanthier, N., Rubbia-Brandt, L., Hadengue, A., & Spahr, L. (2012). Bone marrow mononuclear cell infusion into the liver is associated with liver macrophage expansion in patients with decompendated alcoholic liver disease (ALD). Swiss Medical Weekly, 142(Suppl 194), 14S. https://hdl.handle.net/2078.5/201349 (Original work published 2012)