In vitro produced spores of Glomus versiforme were entrapped in alginate beads and their inoculum potentials evaluated by a biological assay. Encapsulated spores were able to germinate and the regenerated mycelium kept its ability to infect roots under controlled conditions. We estimated to 65.8 the number of beads needed, each containing four spores, to infect 50% of a population of susceptible plantlets. These results show the possibility to encapsulate in vitro produced spores and therefore represent a new kind of high quality inoculum, free of pathogens.
Declerck, S., Strullu, D. G., Plenchette, C., & Guillemette, T. (1996). Entrapment of in vitro produced spores of Glomus versiforme in alginate beads: in vitro and in vivo inoculum potentials. Journal of Biotechnology, 48(1-2), 51-57. https://doi.org/10.1016/0168-1656(96)01396-X (Original work published 1996)