A non-invasive version of the beta-sweat test
(2019) 42nd European Cystic Fibrosis Conference — Location: (United Kingdom) Liverpool (5.June.2019)
Files
WS04-3Anon-invasiveversionofthebeta-sweattest.pdf
Open Access - Adobe PDF
- 48.44 KB
Details
- Authors
- Abstract
- (en) OBJECTIVES: A bioassay for CFTR function describing a ratio between CFTRdependent sweat rate, evoked by a β-adrenergic cocktail, and CFTRindependent sweat rate, induced by a cholinergic agent, was reported in small cohorts using multiple intradermal injections. The aims of this work is to validate a non-invasive version of the image-controlled β-adrenergic sweat test, and to build reference CFTR-dependent/-independent sweat rate ratios in larger cohorts. METHODS: Sweat secretion was initially stimulated by pilocarpine iontophoresis using a Macroduct (Wescor). The stimulated skin areawas covered with an oil layer and sweat droplets were imaged for 10 minutes. A second iontophoresis was followed using atropine, to block the prior cholinergic stimulation, and isoproterenol plus aminophylline, to maximize intracellular cAMP. Images of blue eriogalucine-stained sweat droplets were recorded for 30 minutes. [...]
- Affiliations
Citations
Reynaerts, A., Melotti Paola, Vermeulen François, Gohy, S., Lebecque, P., & Leal, T. (2019). A non-invasive version of the beta-sweat test. Journal of Cystic Fibrosis, 18(Suppl 1), S7. https://doi.org/10.1016/S1569-1993(19)30138-9 (Original work published 2019)