Lytic enzyme-based selective detection of Staphylococcus epidermidis using a porous silicon optical bacteria sensor

Rasson, Jonathan;Nannan, C.;Mahillon,J.;Francis, Laurent
(2020) 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017) — Location: Savannah, USA (22.October.2017)

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  • Rasson, JonathanUCLouvain
    Author
  • Nannan, C.Laboratory of Food and Environmental Microbiology, Université Catholique de Louvain, Belgium
    Author
  • Mahillon,J.Laboratory of Food and Environmental Microbiology, Université Catholique de Louvain, Belgium
    Author
  • Francis, Laurentorcid-logoUCLouvain
    Author
Abstract
In this work, we developed a porous silicon optical bacteria sensor and demonstrated its use for a label-free and selective detection of Staphylococcus epidermidis bacteria. The selective optical detection of bacteria is explored by using a selectivity scheme based on the lytic enzyme lysostaphin to selectively target S. epidermidis instead of using a surface functionalization with antibodies. The detection is performed on a porous silicon sensor, passivated by atomic layer deposition of oxide for an improved stability in aqueous media, using Reflective Interferometric Fourier Transform Spectroscopy.
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Rasson, J., Nannan, C., Mahillon, J., & Francis, L. (2020). Lytic enzyme-based selective detection of Staphylococcus epidermidis using a porous silicon optical bacteria sensor. Proceedings of MicroTAS 2017. Accepted/in-press. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017), Savannah, USA. https://hdl.handle.net/2078.5/168123 (Original work published 2020)