MAINTENANCE EN COURS / SITE UNDER MAINTENANCE

Une opération de maintenance est en cours: les résultats de recherches et les exportations peuvent être incohérent.
Site under maintenance: search & exportation results could be inconsistent.
 

Immobilizing transaminase in a macroporous silica monolith for flow mode enantioselective transamination

Debecker, Damien;van den Biggelaar, Ludivine;Gauchet, Adrien;Khan, Mohammad Shahneawz;Soumillion, Patrice
(2018) International Symposium on Relations between Homogeneous and Heterogeneous Catalysis (ISHHC18) — Location: Sydney, Australia (22.July.2018)

Files

DebeckerISHHCflowtransamination.pdf
  • Open Access
  • Adobe PDF
  • 278.61 KB
Download

Details

Authors
  • Debecker, DamienUCLouvain
    Author
  • van den Biggelaar, LudivineUCLouvain
    Author
  • Gauchet, AdrienUCLouvain
    Author
  • Khan, Mohammad ShahneawzUCLouvain
    Author
  • Soumillion, PatriceUCLouvain
    Author
Abstract
In this communication, we will present the immobilization of TA on silica monoliths that are particularly appropriate for the design of continuous flow reactors. The silica monoliths are prepared by a bottom-up sol-gel method based on emulsion templating. They minimize pressure drop, ensure a plug flow regime, and are easy to manipulate. After presenting the proof-of-concept with a simple flow reactor featuring covalently immobilized TA (ATA-117 from Codexis), we will show how the immobilization of the enzyme – and therefore the catalyst performance – can be optimized by tuning parameters of the monolith surface functionalization. We will show that a self-produced TA of higher purity allows reaching higher production rates. We will also disclose an attempt to run a chiral amine synthesis using a cascade biocatalytic process to shift the thermodynamic equilibrium of the reaction towards the desired product, as proposed by Turner et al., but here in flow mode.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Debecker, D., van den Biggelaar, L., Gauchet, A., Khan, M. S., & Soumillion, P. (2018). Immobilizing transaminase in a macroporous silica monolith for flow mode enantioselective transamination. International Symposium on Relations between Homogeneous and Heterogeneous Catalysis (ISHHC18), Sydney, Australia. https://hdl.handle.net/2078.5/126989