Simultaneous intracellular chloride and pH measurements using a GFP-based sensor
(2010) Nature Methods : techniques for life scientists and chemists — Vol. 7, n° 7, p. 516-518 (2010)
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- Abstract
- Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments.
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Arosio, D., Ricci, F., Marchetti, L., Gualdani, R., Albertazzi, L., & Beltram, F. (2010). Simultaneous intracellular chloride and pH measurements using a GFP-based sensor. Nature Methods : techniques for life scientists and chemists, 7(7), 516-518. https://doi.org/10.1038/nmeth.1471 (Original work published 2010)