Basal lamina characterization in frozen-thawed and long-term grafted human prepubertal ovarian tissue
(2021) Reproductive BioMedicine Online — Vol. 42, n° 5, p. 859-869 (2021)
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- (en) RESEARCH QUESTION: Are there differences in the composition and structure of the basal lamina surrounding follicles in prepubertal versus adult human ovarian tissue? DESIGN: Frozen-thawed human ovarian tissue from six prepubertal and seven adult patients was divided into three fragments in each case: two for non-grafted tissue evaluation and one for long-term xenografting to mice. Collagen IV and laminin expression were investigated by immunohistochemistry before and after grafting. The basal lamina was analysed by transmission electron microscopy on frozen-thawed tissue. RESULTS: In frozen-thawed tissue, collagen IV was significantly less expressed around prepubertal follicles than around adult follicles (primordial, P = 0.02; intermediate/growing follicles, P = 0.03), while laminin was significantly more expressed (primordial, P = 0.03; intermediate, P = 0.01). Collagen IV expression was significantly higher around prepubertal primordial follicles in grafted tissue than in non-grafted tissue, reaching similar levels to those in adult tissue. Ultrastructure analysis showed the basal lamina around follicles in prepubertal frozen-thawed tissue to be rather patchy and thinner than around adult follicles (primordial/intermediate, P = 0.001; primary, P = 0.02). CONCLUSIONS: In frozen-thawed tissue, the basal lamina around prepubertal follicles is less mature than around adult follicles, but it becomes similar in both prepubertal and adult subjects after grafting. Grafting could therefore induce maturation of the basal lamina around prepubertal follicles.
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Philippart, C., Mansciangelo, R., Camboni, A., Donnez, J., & Dolmans, M.-M. (2021). Basal lamina characterization in frozen-thawed and long-term grafted human prepubertal ovarian tissue. Reproductive BioMedicine Online, 42(5), 859-869. https://doi.org/10.1016/j.rbmo.2021.02.012 (Original work published 2021)