Nanonewton forces between Staphylococcus aureus surface protein IsdB and vitronectin
(2020) Nanoscale Advances — Vol. 2, n° 12, p. 5728-5736 (2020)
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- Authors
Mathelie-Guinlet, Marion 
Viela Bovio, Felipe Pietrocola, Giampiero Speziale, Pietro
- Abstract
- Single-molecule experiments have recently revealed that the interaction between staphylococcal surface proteins and their ligands can be extremely strong, equivalent to the strength of covalent bonds. Here, we report on the unusually high binding strength between Staphylococcus aureus iron-regulated surface determinant B (IsdB) and vitronectin (Vn), an essential human blood protein known to interact with bacterial pathogens. The IsdB–Vn interaction is dramatically strengthened by mechanical tension, with forces up to 2000 pN at a loading rate of 105 pN s−1. In line with this, flow experiments show that IsdB-mediated bacterial adhesion to Vn is enhanced by fluid shear stress. The stress-dependent binding of IsdB to Vn is likely to play a role in promoting bacterial adhesion to human cells under fluid shear stress conditions.
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Citations
Mathelie-Guinlet, M., Viela Bovio, F., Pietrocola, G., Speziale, P., & Dufrêne, Y. (2020). Nanonewton forces between Staphylococcus aureus surface protein IsdB and vitronectin. Nanoscale Advances, 2(12), 5728-5736. https://doi.org/10.1039/d0na00636j (Original work published 2020)