Yam (Dioscorea rotundata Poir.) Displays Prezygotic and Postzygotic Barriers to Prevent Autogamy in Monoecious Cultivars

Denadi, Narcisse;Yolou, Mounirou;Dadonougbo, Ayènan Eric;Zoundjihékpon, Jeanne;Quinet, Muriel;et.al.
(2022) Agronomy — Vol. 12, n° 4, p. 872 (2022)

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Authors
  • Denadi, NarcisseUCLouvain
    Author
  • Yolou, MounirouLaboratoire de Génétique Ecologique, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Cotonou 01BP4521, Benin
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  • Dadonougbo, Ayènan EricLaboratory of Biotechnology, Genetic Ressources and Plant and Animals Breeding (BIORAVE), National University of Sciences Technology, Ingeneering and Mathematics, Dassa-Zoumé 01BP14, Benin
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  • Zoundjihékpon, JeanneLaboratoire de Génétique Ecologique, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Cotonou 01BP4521, Benin
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  • Quinet, Murielorcid-logoUCLouvain
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Abstract
Cultivated yam (D. rotundata) is a staple tuber crop in West Africa whose sexual reproduction control remains largely unknown despite its importance for plant breeding programs. In this paper, we compared self-pollination, intracultivar cross-pollination and intercultivar cross-pollination in three monoecious cultivars (Amoula, Heapala and Yassi). Results showed that pollen viability (49%) and stigma receptivity (40%) were similar in monoecious and dioecious plants, suggesting that autogamy could occur in monoecious plants. However, fruit and seed sets were significantly lower after self-pollination compared to cross-pollination. Overall, autogamy reached 11% and pollen lability was almost zero (<1%). The low percentage of pollen grains germinating on the stigma (37%) and pollen tubes reaching the ovules (25%) after self-pollination partly explained the low seed set. Strong inbreeding depression was observed after self-pollination and almost all fruits and about 75% of the seeds resulting from self-pollination showed malformations. Seed germination was also 20 times lower after self-pollination compared to cross-pollination. Sexual reproduction remained low in D. rotundata even after cross-pollination as fruit and seed set did not exceed 18% and 13% respectively. Moreover, comparison between intracultivar cross-pollination and self-pollination revealed intravarietal genetic diversity inside the analyzed yam cultivars. Overall, our results showed that D. rotundata has a very low tolerance to autogamy in monoecious cultivars and has developed pre- and postzygotic mechanisms to limit selfing.
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Denadi, N., Yolou, M., Dadonougbo, A. E., Zoundjihékpon, J., Dansi, A., Gandonou, C., & Quinet, M. (2022). Yam (Dioscorea rotundata Poir.) Displays Prezygotic and Postzygotic Barriers to Prevent Autogamy in Monoecious Cultivars. Agronomy, 12(4), 872. https://doi.org/10.3390/agronomy12040872 (Original work published 2022)