Metabolic signature of C-labeled wheat bran consumption related to gut fermentation in humans: a pilot study.
(2023) European Journal of Nutrition — Vol. 62, n° 6, p. 2633-2648 (2023)
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- Authors
Meiller, Laure 
Sauvinet, Valérie Ranaivo, Harimalala Nazare, Julie-Anne
- Abstract
- PURPOSE: The aim of this pilot study was to analyze concomitantly the kinetics of production of 13C-labeled gut-derived metabolites from 13C-labeled wheat bran in three biological matrices (breath, plasma, stools), in order to assess differential fermentation profiles among subjects. METHODS: Six healthy women consumed a controlled breakfast containing 13C-labeled wheat bran biscuits. H2, CH4 and 13CO2, 13CH4 24 h-concentrations in breath were measured, respectively, by gas chromatography (GC) and GC-isotope ratio mass spectrometry (GC-IRMS). Plasma and fecal concentrations of 13C-short-chain fatty acids (linear SCFAs: acetate, propionate, butyrate, valerate; branched SCFAs: isobutyrate, isovalerate) were quantified using GC-combustion-IRMS. Gut microbiota composition was assessed by16S rRNA gene sequencing analysis. RESULTS: H2 and CH4 24 h-kinetics distinguished two groups in terms of fermentation-related gas excretion: high-CH4 producers vs low-CH4 producers (fasting concentrations: 45.3 ± 13.6 ppm vs 6.5 ± 3.6 ppm). Expired 13CH4 was enhanced and prolonged in high-CH4 producers compared to low-CH4 producers. The proportion of plasma and stool 13C-butyrate tended to be higher in low-CH4 producers, and inversely for 13C-acetate. Plasma branched SCFAs revealed different kinetics of apparition compared to linear SCFAs. CONCLUSION: This pilot study allowed to consider novel procedures for the development of biomarkers revealing dietary fiber-gut microbiota interactions. The non-invasive assessment of exhaled gas following 13C-labeled fibers ingestion enabled to decipher distinct fermentation profiles: high-CH4 producers vs low-CH4 producers. The isotope labeling permits a specific in vivo characterisation of the dietary fiber impact consumption on microbiota metabolite production. CLINICAL TRIAL REGISTRATION: The study has been registered under the number NCT03717311 at ClinicalTrials.gov on October 24, 2018.
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Citations
Meiller, L., Sauvinet, V., Breyton, A.-E., Ranaivo, H., Machon, C., Mialon, A., Meynier, A., Bischoff, S. C., Walter, J., Neyrinck, A., Laville, M., Delzenne, N., Vinoy, S., & Nazare, J.-A. (2023). Metabolic signature of C-labeled wheat bran consumption related to gut fermentation in humans: a pilot study. European Journal of Nutrition, 62(6), 2633-2648. https://doi.org/10.1007/s00394-023-03161-5 (Original work published 2023)