CuO grown by room-temperature direct current (DC) reactive magnetron sputtering is introduced to realize p-type thin-film transistors (TFTs), with a high-k HfO2 gate dielectric fabricated by atomic layer deposition (ALD). The devices work in accumulation mode (AM) with two apparent threshold voltages corresponding to the formation of buried channel and accumulation layer, respectively. CuO AM TFT with a channel length of 25 μm exhibits competitive on-off ratio (Ion/Ioff) of 1.3x10², subthreshold swing (SS) of 1.04 V dec-1, and field-effect mobility (µFE) of 1.1x10-3 cm2 V-1 s-1 at room temperature. By measuring a CuO metal oxide semiconductor (MOS) capacitor at room temperature, a high acceptor doping density (NA) of ~5x1017 cm-3, a high positive effective fixed surface charge density (Qf) of ~91012 cm-2 and a low interfacial trap charge density (Dit) of ~6x1010 eV-1 cm-2 at the HfO2/CuO interface are estimated. The µFE extracted from the accumulation regime appears lower than the Hall mobility measured for a similarly processed CuO layer on glass due to the increased hole concentration in CuO TFT, compared to a Hall concentration of ~1014 cm-3, following MOS process. SS appears limited by the decreased channel to gate capacitance (Ccg) related to the buried channel in AM TFTs, parasitic capacitance to ground and potentially very high interfacial traps at the non-passivated CuO/air interface.