In the sartorius muscle of Rana temporaria isometrically tetanized at 0°C, the ratio hA / hB is 1.2 for the first tetanus; it falls to about 0.5 if the second tetanus follows after 5 to 10 s, and still reaches only 0.8 if the interval is increased to two minutes. However, the release of the second tetanus is always slower. Return to normal requires more than 15 minutes at 0°C.
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Aubert, X., & Maréchal, G. (1963). [The labile fraction of thermogenesis associated with the maintenance of isometric contraction]. Archives Internationales de Physiologie et de Biochimie, 71(2), 282-283. https://hdl.handle.net/2078.5/215381 (Original work published 1963)