beta-Carotene is incorporated or mobilized along with triglycerides in bovine adipose tissue in response to insulin or epinephrine
(2009) Journal of Animal Physiology and Animal Nutrition (Print) — Vol. 93, n° 1, p. 83-93 (2009)
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- Pasture fed cattle ingest substantial amounts of beta-carotene (beta-C). Not all of the carotenoid compound is transformed into vitamin A, but the surplus is deposited in adipose tissue (AT). The mechanisms of beta-C incorporation and mobilization are unknown. Two experiments were conducted using explants from bovine AT cultured in vitro. First, beta-C incorporation by explants from three animals was examined with different beta-C concentrations (0, 1, 5 and 20 mu m) and different times of incubation (every 5 h up to 25 h). The data showed a significant increase of beta-C concentration in explants only for 20 mu m beta-C. Secondly, effects of insulin and epinephrine on beta-C and triglyceride (TG) contents of explants were studied. Explants from six animals were incubated with either hormone and 0 or 20 mu m beta-C for 20 h. Both TG and beta-C contents were affected positively by insulin and negatively by epinephrine. Interestingly, changes in ratios of beta-C/TG (hormone vs. control) were similar (1.7 x 10(-3) and 1.8 x 10(-3)), respectively, for insulin and epinephrine, indicating that beta-C level is directly related to TG content. We also report the presence of mRNA for beta-C 15, 15' oxygenase in bovine AT. The in vitro culture system using explants from bovine AT is a promising model to investigate factors that might affect the accumulation and metabolism of beta-C.
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Arias, E., Gonzalez, A., Shimada, A., Varela-Echavarria, A., Ruiz-Lopez, F., During, A., & Mora, O. (2009). beta-Carotene is incorporated or mobilized along with triglycerides in bovine adipose tissue in response to insulin or epinephrine. Journal of Animal Physiology and Animal Nutrition (Print), 93(1), 83-93. https://doi.org/10.1111/j.1439-0396.2007.00783.x (Original work published 2009)