Amine-reactive ova multimers for auto-vaccination against cytokines and other mediators: Perspectives illustrated for GCP-2 in l. major infection
(2011) Journal of Leukocyte Biology — Vol. 89, n° 6, p. 1001-1007 (2011)
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- Abstract
- Anticytokine auto-vaccination is a powerful tool for the study of cytokine functions in vivo but has remained rather esoteric as a result of numerous technical difficulties. We here describe a two-step procedure based on the use of OVA multimers purified by size exclusion chromatography after incubation with glutaraldehyde at pH 6. When such polymers are incubated with a target protein at pH 8.5 to deprotonate reactive amines, complexes are formed that confer immunogenicity to self-antigens. The chemokine GCP-2/CXCL6, the cytokines GM-CSF, IL-17F, IL-17E/IL-25, IL-27, and TGF-β1, and the MMP-9/gelatinase B are discussed as examples. mAb, derived from such immunized mice, have obvious advantages for in vivo studies of the target proteins. Using a mAb against GCP-2, obtained by the method described here, we provide the first demonstration of the major role played by this chemokine in rapid neutrophil mobilization after Leishmania major infection. Pre-activated OVA multimers reactive with amine residues thus provide an efficient carrier for auto-vaccination against 9-90 kDa autologous proteins. © Society for Leukocyte Biology.
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Uyttenhove, C., Marillier, R. G., Tacchini-Cottier, F., Charmoy, M., Caspi, R. R., Damsker, J. M., Goriely, S., Su, D., Van Damme, J., Struyf, S., Opdenakker, G., & Van Snick, J. (2011). Amine-reactive ova multimers for auto-vaccination against cytokines and other mediators: Perspectives illustrated for GCP-2 in l. major infection. Journal of Leukocyte Biology, 89(6), 1001-1007. https://doi.org/10.1189/jlb.1210699 (Original work published 2011)