Development of a sensitive workflow to analyse T cell phenotype and specificity in patients with cancer or autoimmune diseases
(2019)
Files
Orian_Bricard_Manuscrit_190701.pdf
Open Access - Adobe PDF
- 11.07 MB
Details
- Authors
- Supervisors
- Coulie, Pierre
- Abstract
- Little is known about anti-tumor T cell activity in low antigenic but frequent tumor such as breast carcinoma. We first explored the immunogenicity towards CD8 T cells for 6 primary human breast carcinomas. We screened tumor derived T cell clones for the recognition of tumor specific antigens. We found only 7 T cell clones that were able to recognize mutations. They originate from the same tumor. This demonstrates that anti-tumor T cell activity is possible in primary human breast carcinoma but seems to be rare. Aware of potential selection bias linked to the ability of T cells to grow in vitro and eager to characterize the ex vivo phenotype of the identified anti-tumor T cells, we developed a workflow involving single cell RNAseq analysis of freshly extracted tissue T cells followed by TCR reconstruction in a reporter cell line to evaluate their specificity. This workflow has also been applied to analyze kidney infiltrating T cells from lupus nephritis suffering patients.
- Affiliations
Citations
Bricard, O. (2019). Development of a sensitive workflow to analyse T cell phenotype and specificity in patients with cancer or autoimmune diseases. https://hdl.handle.net/2078.5/62170