Magnetic nanoparticle-enhanced PCR for the detection and identification of Staphylococcus aureus and Salmonella enteritidis.
(2017) The New Microbiologica : quarterly journal of microbiological sciences — Vol. 40, n° 3, p. 165-169 (2017)
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- This paper evaluated magnetic nanoparticle-enhanced PCR for the detection and identification of Staphylococcus aureus and Salmonella enteritidis. Two different types of magnetic nanoparticles designated MPIO (iron concentration 2.5 mg/ml, size 1 μm) and NP (iron concentration 8.7 mg/ml, size 60 nm), both conjugated with S. aureus or S. enteritidis antibodies were evaluated as an enrichment procedure for PCR-detection of the pathogens in Trypticase Soy Broth, milk, blood and meat broth. Bacterial suspensions (1.5 x 108 cfu/ml) were prepared and serial diluted 10-1. The MPIO and NP nanoparticles were added, followed by incubation for 1 hour at room temperature, magnetic separation of the pellet, DNA extraction and PCR, targeting the femA and invA sequences. The nanoparticle-free and the NP-supplemented dilutions were positive down to the 1.5 x 102 cfu/ml concentration for both bacteria. The MPIO-supplemented dilutions were positive down to approx. 2 x 100 cfu/ml concentration, respectively. Bacteria-free TSB was negative by PCR. MPIO nanoparticles (size 1 μm) enhanced the detection of S. aureus and S. enteritidis by PCR, whilst NP nanoparticles (size 60 nm) did not, thus indicating that the size of the magnetic nanoparticles play a significant role in the enrichment procedure.
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Houhoula, D., Papaparaskevas, J., Zatsou, K., Nikolaras, N., Malkawi, H. I., Mingeot-Leclercq, M.-P., Konteles, S., Koussisis, S., Tsakris, A., & Charvalos, E. (2017). Magnetic nanoparticle-enhanced PCR for the detection and identification of Staphylococcus aureus and Salmonella enteritidis. The New Microbiologica : quarterly journal of microbiological sciences, 40(3), 165-169. https://hdl.handle.net/2078.5/38624 (Original work published 2017)